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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°-20°C
- 保质期:
1-3年
- 库存:
999
- 供应商:
亦高生物
- CAS号:
14936-97-1
- 规格:
248266-1G

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文献和实验and resuspended in protein-free medium or salt solution prior to counting.Supplies & Equipment:Eppendorf tubes (1.5 ml)Micropipet (10µl)HemocytometerReagents:HBSS (Hanks' Balanced Salt Solution)sterile Trypan blue solution 0.4% (Sigma T-8154)Procedure:Prepare
(Hanks' Balanced Salt Solution) sterile Trypan blue solution 0.4% (Sigma T-8154) Procedure: Prepare a cell suspension in HBSS Transfer into Eppendorf tube: 0.5 ml of 0.4% Trypan blue solution 0.3 ml
Methylene Blue DNA staining protocol
Methylene Blue DNA staining protocol Protocol: Load 2-5X the amount of DNA that would give bands of moderate intensity on an ethidium bromide stained gel. Typically this is something on the order of 0.5-2.5 µg
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