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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°-20°C
- 保质期:
1-3年
- 库存:
999
- 供应商:
亦高生物
- CAS号:
10025-76-0
- 规格:
238066-1G

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文献和实验Replication timing by density transfer
-Aldrich (previously from Isotec, which is now part of Sigma-Aldrich) "-N" medium =1.61 g yeast nitrogen base w/o amino acids and ammonium sulfate, 11.1 g succinic acid, 6.67 g NaOH per litre. Pronase : We use
Preparation of BAC DNA with by Alkaline Prep Method
CLONE ISOLATION PREP - PROTOCOL Before you begin isolating DNA from the cultures: --GENTLENESS is key through step 10! --Try to do prep steps 1-8 at the side of the centrifuge --Make Solution III and place in fridge or on ice morning
pH 8.0 25 ml 1M Tris pH 8.0 10 mM EDTA 20 ml 0.5 M EDTA pH 8.0 up to 1 liter with Q store at 4 ℃ Solution II 1% SDS 2.5 ml 20% SDS 0.2N NaOH 1.0 ml 10N NaOH up to 50 ml with Q make fresh as needed Solution III 25% potassium acetate 250 g
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