- ￥674
- Sigma
- USA
- P8043-100MG
- 2025年11月07日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C-20°C
- 保质期：
1-3年
- 库存：
999
- 供应商：
亦高生物
- 规格：
P8043-100MG
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文献和实验A safe method of extracting DNA from Coccidioides immitis
nitrogen, lyophilized, and ground with a mortar and pestle. About 10 mg of dry ground tissue is mixed with 400 ul lysis buffer (50 mM Tris, 100 uM NaCl, 5 mM EDTA, 1 sodium dodecyl sulfate), and incubated at 80 C for 10 min., cooled to 40 C and incubated
Single tube confirmation PCR protocol
containing 60 U/ml of Zymolyase (This solution is generated by resuspending 6 mg of 100T Zymolyase in 10 mls of water. Ordering information: Seikagaku Corp., #120493-1). - Sterile yellow tips work great for this task. - Repeat this step
Deglycosylation of Glycoproteins Using Endoglycosidases
(CHO)-N-S-D-I-S-S-T-R IGM, biantennary with core 1-6 fucose, Sodium acetate 100 mM pH 4.5 Protocol: 1. Heat denature transferrin by incubation of 1 mg/ml of the glycoprotein in 0.5% SDS for 3 min. at 90°C.
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