- ￥119
- Sigma
- USA
- 89951-200UL-F
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C-20°C
- 保质期：
1-3年
- 库存：
999
- 供应商：
亦高生物
- 规格：
89951-200UL-F
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文献和实验Materials 1. PBS/BSA: PBS solution A with 1% BSA (Boehringer Mannheim 100 350) and 0.1% NaN3 , stored at 4o C. Bring to room temperature before use. 2. Primary antibody, diluted appropriately with PBS/BSA. Need 200 ul per 45x50 mm coverslip
Basic Fluorescent in situ Hybridization (FISH)
is labeled with biotin-11-dUTP using nick translation random priming or the polymerase chain reaction Hybridization mix solution: 50% formamide [ Details ： Product No. F7508], 10% dextran sulfate [ Details ： Product No. D8906], 0.1% SDS
in 0.5ml of blocking solution containing the fluorescent labeled anti-DIG or anti-Biotin antibodies for 1 hour at RT (described in Solutions and antibodies for detection of the FISH signals). For two-colour FISH, incubate first with the anti-DIG
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