Sartorius赛多利斯VC1061Vivaspin 10

超滤膜

率高5,000 MWCO0.24肽的回收率高，流速较快10,000 MWCO0.41应用范围广，流速快，吸附低30,000 MWCO0.41应用范围广，流速快50,000 MWCO0.45精确的截留分子量限100,000 MWCO0.35免疫球蛋白的收率高三醋酸纤维素，pH范围4 ~ 8 5,000 MWCO0.04去除肽和蛋白10,000 MWCO0.11微小组分，游离/结合药物研究20,000 MWCO0.58样品清洗，HPLC样品制备再生纤维素，pH范围3 ~ 11 10,000 MWCO0.18

Qiagen MIDI protocol

in the Beckman. Promptly pour supernatant into another round-bottom 13 ml polycarbonate tube and re-centrifuge at 20,000 x g for 15 min. While the centrifugation is happening, equilibrate a Qiagen-tip 100 (see next step). 7. To equilibrate the tip

Replication timing by density transfer

, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16