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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310674163
中文名称:
英文名称:X2 Cell factory Nunc 40 tray active gassed (pack of 2)
货期:热销品现货,其他请咨询




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文献和实验ES / MEF cell culture and electroporation of targeting construct
of cells to a microfuge tube containing the targeting construct (40ug of clean linear DNA in 1 X TE @ 1 ug/ul for each electroporation). Mix the cells and DNA with a 200-1000 ul barrier tip trying not to create bubbles. Add 400 ul of the cell/DNA mixture
Recipes for Tissue Culture Selective Drug Stocks
Recipes for Tissue Culture Selective Drug Stocks 100X G418 (Geneticin): Obtained as crystalline powder from GIBCO (cat.# 860-1811) and stored @ room temp. in T932. The amount of active ingredient per total weight is printed
ES / MEF cell culture and electroporation of targeting construct
(40ug of clean linear DNA in 1 X TE @ 1 ug/ul for each electroporation). Mix the cells and DNA with a 200-1000 ul barrier tip trying not to create bubbles. Add 400 ul of the cell/DNA mixture to a BTX cuvette. Set Electroporator as follows: 500
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