赛默飞世尔Thermo Fisher GLD GAUZ 10

In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes

Solution: 100 mM NaCl; 5 mM MgCl2 ; 100 mM Tris (pH 9.5); 0.1% Tween 20; 1mM Levamisole. Levamisole is a potential inhibitor of endogenous phosphatases. Culture tubes: Available from Fisher as 6 mm (ID) x 50 mm (length) in either Pyrex or borosilcate

常用缓冲液配制－2

into several small screw-cap jars (~50 ml capacity) Store at room temperature  20 x Energy Regeneration System* 150 mM creatine phosphate (Boehringer #127574) 2 ml of 100 mM MgATP Stock (20 mM ATP, 20 mM MgSO4 ) 40ml 0.5 M EGTA (2 mM EGTA)

【共享】某机构发表的核酸相关研究的文章（EST,SNP等），PDF全文下载

, Noegel AA, Insall RH (2005) The Dictyostelium genome encodes numerous RasGEFs with multiple biological roles Genome Biol 6: R68 pdf Winckler T, Szafranski K, Glöckner G (2005) Transfer RNA gene-targeted integration: an adaptation