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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
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- 保修期:
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EA
货号:T_701DX064298
中文名称:
英文名称:PROSWIFT RP-3U 4.6X50MM
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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Transfection of Fibroblasts - SuperFect 1) Seed cells in mid- to late afternoon and let grow overnight. NIH3T3 2x105/well of 6-well dish 6x105/6cm plate REF52 1x105/well of 6-well dish 2.5x105/6cm plate WI
In Situ HYBRIDIZATION TO TISSUE SECTIONS
1.0ml 10mM rGTP 1.0ml 0.5mM rUTP 1.0ml 0.75M DTT 1.0ml RNase-Block (25U/ml) 2.5ml 35S-rUTP (100mCi) 1.0ml T3 or T7 polymerase (10U) Vortex, spin down and incubate 1-2h @ 30 degrees C. Incorporation should be 70-90%. Determine
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