赛默飞世尔Thermo Fisher X50 LENS CL
EANING TISSUES 95MM X 135mm (1 x pack/envelope containes 50 sheets) 货号：T_70311517362- ￥5 - 100
- Thermo Fisher
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- T_70311517362
- 2025年07月12日
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- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311517362
中文名称：
英文名称：X50 LENS CLEANING TISSUES 95MM X 135mm (1 x pack/envelope containes 50 sheets)
货期：热销品现货，其他请咨询
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
Protein extraction from whole tissues for IEF
of methanol 80% acetone (100 ml) - store at -20 C 70% ethanol (100 ml) - store at -20 C IEF Sample Buffer (10 ml) 8 M urea 4.85 g 2 M thiourea 1.52 g 2% CHAPS 0.2 g 2% Triton X-100 200 ul 50 mM DTT 8 mg/ml make to 10 ml
Protocols for protein extraction from whole tissues for isoelectric focusing
at 5000 g for 15 min and dry pellet by inverting on Kimwipe for 15 min at37℃ 9.Resuspend final pellet in 0.5-1 ml of IEF extraction solution (8 M urea,2 M thiourea,2% CHAPS,2% Triton X-100,50 mM DTT,0.5% pH 3-10 ampholytes)by pipetting and vortexing
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