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999
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北京泽平
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货号：T_70311744203
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英文名称：X100 FILTER PAPER GR114 150MM 3µM
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文献和实验antibody: Anti-mouse IgG-Alkaline Phospphatase Buffers Lysis buffer: 100ml 50 mM Tris-Cl, pH 8.0 0.6057g 150m M NaCl 0.8766g 0.02%NaN3 0.02g 100ug/mlPMSF 0.01g 10ug/ml Aprotinin 0.001g 1% Triton X100 1g 2x SDS-loading buffer: 100ml 100 mM Tris
A quick RNA mini-prep for Neurospora mycelial cultures
is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C
Southern Hybridization Protocols
in a hybridization, denature the labelled DNA by heating to 95-100 ℃ for 5 min, then chilled on ice.Add 50 m l probes to each hybridization tubeHybridize o/n at 65 ℃Filter washing: preparation of washing solutionW1 = 2SSC-0.5% SDS —> 150ml kept at RT W1 = 0.5SSC
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