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999
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北京泽平
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货号：T_70103-395-450
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英文名称：CRYO CELL DIVIDERS 25 CELL
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
The technique described here is a slight modification (March 1997) of methods presented in: Nagy A., J. Rossant. 1993. Production of completely ES cell-derived fetuses. In : Gene Targeting: A Practical Approach. (ed. A. Joyner). IRL Press at Oxford
Freezing and Thawing of Mammalian Cell Lines
For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture. Freezing Preparation Cells are to be frozen in liquid nitrogen, so make sure
- Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells
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