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999
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北京泽平
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货号:T_70119-156-587
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英文名称:CLEAN ROOM/ISO 8/24X36
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文献和实验Plant DNA methylation analysis by bisulfite genomic sequencing
) at temperature appropriate for used restriction enzyme; Add 0.5ml Tris-buffer-saturated phenol-chroroform, vortex; Centrifuge with Eppendorf 5414 or equivalent at 10000 x g for 2 minutes; Transfer the upper aqueous phase to clean 1.5ml
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
° (a 36 hr hybridization may give increased signal and lower background than 15 hr). 17) Draw off and save hybridization solution. Wash 2 x 2 min in HB at 48°. 18) Wash 4 x 30 min in HB at 48°. 19) Wash: 1 x
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