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999
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北京泽平
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货号：T_70116-317-40
中文名称：
英文名称：RAPIDVAP N2 FOR 600ML TUBES
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C
Cool growth competent E . coli
, while swirling, to final 7 % (~1.4 ml). -Place on ice, 10 min. Dispense into freezing tubes (~0.5-1 ml ea) -Freeze in liquid N2; transfer to -80°. 1 liter SOB: 20 g bactotryptone, 5 g bacto-yeast extract, 0.5g NaCl Dissolve all in 950 ml ddH
A quick RNA mini-prep for Neurospora mycelial cultures
x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C. A 50 ml culture typically yields enough RNA
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