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货号：T_70102-924-926
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英文名称：ROLLER APPARATUS 30POS.
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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Electroporation of P. aeruginosa
on a roller. Transfer 2 ml of the overnight culture to 200 ml of fresh L-broth in a 1-liter sidearm flask. Grow this culture at 37°C with shaking (200 rpm) until OD540 = 0.3-0.5. Aliquot 35-40 ml
Steve HahnLast modified10/16/98Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases .1. Use PAGE
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