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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311701963
中文名称：
英文名称：FILTER HOLDER W/RESEVOIR 47MM SS
货期：热销品现货，其他请咨询
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文献和实验Southern Hybridization Protocols
8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml16.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then added to the solution at 65
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
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