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货号：T_701AA33210ED
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英文名称：AL23 TUBE FOR HTNG C 5.0 500MM
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
interest in chromatin long-range interactions. RELATED INFORMATION The 6C assay combines three different methodologies: chromosome conformation capture (3C) (Dekker et al. 2002), chromatin immunoprecipitation (ChIP), and cloning (Fig. 1 ). The first step
A quick RNA mini-prep for Neurospora mycelial cultures
is thus desirable. Such a system has been developed for isola-ting plant RNA (Nagy et al. 1988 Plant Molecular Biology Manual, B4; ed. Gelvin and Schilperoort, Klewer Academic Publishing, pp. 1-29), and we have adapted this procedure for use with Neurospora
solution and remove by aspiration. Next,add 1 mL of trypsin and incubate at 37°C for 2–3 min. 2. To the trypsinized cells, add 10 mL of complete RPMImedium, transfer to a 15-mL snapcap polypropylene tube,and centrifuge at 500× g at 4°C for 5 min
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