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- 技术资料
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999
- 供应商：
北京泽平
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- 保修期：
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货号：T_70310233143
中文名称：
英文名称：TEMPERATURE PROBE
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文献和实验Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
temperature. 4. Add 1 ml of Cell Dissociation Buffer. Incubate for 2 minutes at room temperature. 5. Add 6 ml of Serum Containing Medium. 6. Triturate the cells vigorously until a single-cell suspension is achieved. 7. Count the cells
in the vacuum oven for one hour to crosslink the DNA to the membrane. The dried membrane is stable at room temperature. The membrane can be stored dry between two sheets of GB002 in a plastic bag at 23-25℃. Preparation of Radiolabeled Probe Note: Wear lab
Southern Hybridization Protocols
containing Hybaid tube. Rotate tube for 4 to 5 hours at 60℃ or 65℃ before adding the denatured radioactively labelled probe. Continue with hybridization overnight with the same solution and temperature. Note: The prehyb- and hybridization
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