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货号:T_70310721483
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英文名称:TWO-STOP TUBING DIA 2.8 MM mm)
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文献和实验Procedures for In Vitro Production of Bovine Embryos
and oocytes. From left to right are 1) a 1 cc syringe with an extension of rubber tubing connected to a Unopette , 2) a wiretrol (from Drummond Scientific; we purchase from Fisher ), 3) the same device as #1 without the rubber tubing extension and 4) a 5
V294.PART II,Chapter 5 细胞粘附实验(英文版)
). 7. Stop solution: 5 mM ethylene diamine tetraacetic acid (EDTA), 50 mM glycinebuffer, pH 10.4. 2.2. Measurement of Adhesion in Shear Stress 1. Cultured endothelial cells or substrate coating materials (fibronectin, gelatin, orother extracellular
7.4, 10 mM MgCl2, 1 mM DTT The kinase reactions are performed in a volume of 20 μg containing 1 μM cold ATP and 5 μCi gamma-32P-ATP and 0.5 μg GST-Crk in kinase buffer. Incubate the kinase reactions for 40 min. at room temp. Stop reaction by adding
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