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- 详细信息
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- 技术资料
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999
- 供应商：
北京泽平
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热销品大量现货，其余请咨询
- 保修期：
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货号：T_701AA30062DW
中文名称：
英文名称：AL23 TUBE (1 END CLSD) 2 300MM
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文献和实验- normally blunt-end ligation of insert to vector does not reform the restriction site (therefore when you design the oligo that make sure it doesn't reform the restriction site when ligated), therefore only religated vector is digested. See one-tube PCR
the restriction enzymes well. g) Keep the buffer in small aliquots as the freezing/defrosting cycles may damage the ATP. Also do not use too high a concentration of ATP (max. 0.5mM) for blunt-end ligation as polyadenation of blunt-ended may occur? h) The ligase
it doesn't reform the restriction site when ligated), therefore only religated vector is digested. See one-tube PCR cloning for an example. k) Do remember to polish off the ends after PCR if you're doing blunt-end ligation of PCR product produced by Taq
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