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- 文献和实验
- 技术资料
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999
- 供应商：
北京泽平
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- 保修期：
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EA
货号：T_7011501-25
中文名称：
英文名称：BUFFER 4.00, RICCA, RED, 10 L
货期：热销品现货，其他请咨询
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文献和实验Recombineering/Lambda red-mediated gene replacement
Spin at 2500rcf for 10 minutes at 4°C Decant supernatant, discard Resuspend each pellet in 1.25 mL ice cold transformation buffer Combine resuspended pellets in single tube Remove 400 μL for immediate
in Tris�HCl buffer (10 mM, pH 8.5). Approximately 3 μg of each linear BAC was co-transformed to Red/ET[hygro] electrocompetent bacteria and selection of positive clones was initially performed on Stp/Cm agar plates and further in liquid LB media (Stp/Cm
Purification of recombinant sBRF M166L
J.Movius,K,Coachman,and S.Hahn (Hahn Lab) last modified 10/9/00 Induction of BRF in bacteria and purification on Ni-NTA agarose Transform BRF plasmid into strain BL21 DE3 (pLysS)or JM25 (DE3 containing the Arg tRNA over expression plasmid
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