赛默飞世尔Thermo Fisher 可重复使用A级美标大肚

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

anddiluted further to 20 mM as working solutions (see Table 1). Store at -20°C. 12. Agarose powder. 13. Ethidium bromide at 10.0 mg/mL (Invitrogen). Table 1 Sequence (5‘→3') of oligonucleotides used in STA-PCR and SplinkBlunt-PCR  

RNA purification using TRizol--TRIZOL法提取RNA

to a fresh tube. b.Add 0.5 ml of isopropyl alcohol to precipitate RNA. If this RNA will be used for RT-PCR, first add 50 μl isopropyl alcohol, mix, incubate the samples at room temperature for 5 minutes and centrifuge at 12,000 x g for 10 minutes at 4oC

Beta-gal Assay

, or dilute cell mix 10x or 20x (40 or 80 brought to 0.8mL with Z-buffer). 5. Using Pasteur pipet, add 1 drop of 0.1% SDS and 2 drops of chloroform to each tube. 6. Vortex well for 15 sec. 7. Equilibrate @ 30 deg C for 15 min. 8. Add 160