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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70312091712
中文名称：
英文名称：JOINT,FLAT FLANGE,100MM BORE
货期：热销品现货，其他请咨询
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文献和实验Alkaline Lysis and CsCl Purification of BAC DNA for Transgenic Production.
lysis efficiency), resuspend pellet from each 250ml culture in 10 ml of Solution I by pipeting up and down with 10ml stripette: Let stand for 5 minutes at room temperature. Solution I 10mM EDTA, pH 8.0 for 100ml bring up 2ml of 0.5M EDTA
Immunostaining of Paraffin Sections
slides in 80, 90 and 2x 100 EtOH (1 min each), then immerse in 2 changes of xylene (1 min each). Mount cover slips on the slides using Permount (Fisher) and examine in the light microscope.
Southern Analysis Procedure (for analysis of genomic DNA or ordering of clones): 1) Prepare genomic DNA (ref.p.9.22, Maniatis) from cultured cells using one T75 flask (wash two times with PBS and then use a rubber policeman to release), or cells
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