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文献和实验【转贴】immunofluorescence staining
well for many other cell lines. However, some cells may require adjustments in length of incubations, and concentration of formaldehyde or Triton X-100.Part 1: Initial Fixation, with or without extractionAspirate or pipette off media. Wash X 2 with ice-cold PBS (containing
Differential staining of trophectoderm and inner cell mass in combination with TUNEL labeling of bovine embryos将滋养层和内细胞群分别染色，TUNEL检测牛胚胎凋亡 Simple protocol for differential staining/TUNEL analysis of bovine
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
(PSG). Do not remove the laminin from the wells until immediately before use. Differentiation 16. Add 100 µL of NEP complete medium to each well of a poly-D-lysine/laminin-coated eight-well chamber slide (prepared
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