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999
- 供应商：
北京泽平
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热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_7010340920B
中文名称：洗瓶
英文名称：W/B VRTK ETOH LDPHE 500ML 4/PK
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文献和实验BrdU-Immunoperoxidase Staining of Paraffin Tissue Sections
substrate, (make fresh): 25 mL 50 mM Tris pH8, 1 mL DAB (3,3-diaminobenzidine, 25 mg/mL stock, -20ºC), 100 µL NiCl2 (10% w/v stock, 4ºC), 10 µL 30% H2 O2. Counter stains: Methyl Green or dilute Eosin (1/4x in 80% EtOH) BrdU Labeling 1. Inject
Bromodeoxyuridine Immunohistochemistry
, add 1 drop of B, vortex, and let sit 30 min. RT. 15. 1X PBS, 3 min.X 5, RT. 16. ABC, 30 min., RT. 17. 1X PBS, 3 min. X 3. 18. pH 7.5 buffer, 15 min., RT: 1 drop pH 7.5 buffer in 2.5 ml house distilled water Do not use ddH2O and PBS
In Situ HYBRIDIZATION TO TISSUE SECTIONS
glass tube -Spot 10ml on a GF/C filter disk -To the remaining 90ml, add 5ml cold 10% TCA; incubate 10' on ice -Filter onto GF/C, wash with 10% TCA, wash with 95% EtOH and dry -20-50% incorporation is good and corresponds to a S.A. ‰ 1-2 x 108
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