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文献和实验Procedures for In Vitro Production of Bovine Embryos
and oocytes. From left to right are 1) a 1 cc syringe with an extension of rubber tubing connected to a Unopette , 2) a wiretrol (from Drummond Scientific; we purchase from Fisher ), 3) the same device as #1 without the rubber tubing extension and 4) a 5
Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor
or commercial NO (Fisher) Methyl red 0.1 M sodium phosphate buffer, pH 7.40 ( appendix 2A
In Situ HYBRIDIZATION TO TISSUE SECTIONS
1.0ml 10mM rGTP 1.0ml 0.5mM rUTP 1.0ml 0.75M DTT 1.0ml RNase-Block (25U/ml) 2.5ml 35S-rUTP (100mCi) 1.0ml T3 or T7 polymerase (10U) Vortex, spin down and incubate 1-2h @ 30 degrees C. Incorporation should be 70-90%. Determine
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