赛默飞世尔Thermo Fisher CAP SCREW O

Northern Blotting方法(Howell Lab)

inverted because the RNA pellets on the bottom. (DNA can be recovered from the CsCl layer.) Cut off the tube bottoms (keep inverted) and resuspend the pellet in 100 µl 0.3 M NaOAc (pH 6) buffer. Transfer to a screw-cap 1.5 ml Eppi. Wash tube

常用缓冲液配制－2

into several small screw-cap jars (~50 ml capacity) Store at room temperature  20 x Energy Regeneration System* 150 mM creatine phosphate (Boehringer #127574) 2 ml of 100 mM MgATP Stock (20 mM ATP, 20 mM MgSO4 ) 40ml 0.5 M EGTA (2 mM EGTA)

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

covalently bound through a 3′‐O ‐hemisuccinate linker (Glen Research) 4‐mL screw‐cap glass vials (Fisher) 5 µm Supelcosil LC‐18