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文献和实验Extraction of RNA from Frozen Sections
that are always handled while wearing gloves. Similarly, all chemicals used for RNA should be dedicated for that purpose and handled with gloves to avoid contamination. 1. Prepare a scintillation vial (Fisher #3-341-13) for each sample
RNA Extraction from Frozen Tissue Sections
. Similarly, all chemicals used for RNA should be dedicated for that purpose and handled with gloves to avoid contamination. 1. Prepare a scintillation vial (Fisher #3-341-13) for each sample containing 1.5 ml of Trizol (Invitrogen#15596-026). Exercise proper
. Molecular Probes; Catalog No. T-20932). See Note 3 for advice on when to use the reagents described in 4 and 5. 6. 100x DAPI (4’,6-diamidino-2-phenylindole) solution (0.1 mg/mL). 2.3.3 Storage, Mounting and Viewing of Samples
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