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货号:T_70312453628
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英文名称:Assem amber target i-d kit, t/s septa, 100/Cs MA: C4010-17AW
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文献和实验Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
). 15. Wickramasinghe, N.S., et al. Estradiol downregulates miR-21 expression and increases miR-21 target gene expression in MCF-7 breast cancer cells. Nucleic Acid Res. , 37-2584 (2009). 16. Ciaudo, C., et al. Highly dynamic and sex-specific
used for PCR in this protocol. 4. Cloning enzymes: FastDigest Bbs I, FastDigest Age I, FastAP(Thermo Scientifi c/Fermentas, Pittsburg, PA, USA), Plasmid-Safe exonuclease (Epicentre, Madison, WI, USA), T7 DNA ligase. 5. 10× T4 DNA ligase buffer
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
7.0 µl 10x Taq Buffer 2.5 µl 25 mM MgCl2 1.5 µl 10 x dNTP mix 5.0 µl Primer * (30 ng/µl) 5.0 µl Digested DNA (100-200 µg/ml) 2.0 µl mineral oil 40 µl * For cDNAs cloned in Bluscript, we use the following primers (21mers): "T
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