赛默飞世尔Thermo Fisher BUFFER, RIC

Purification of mAb (IgG)

7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml

原创：western protocol

buffer: 1 L 30.3 g Trizma base (= 0.25 M) 144 g Glycine (= 1.92 M) pH should be 8.3; do not adjust To make 1L of 1x Blotting buffer: 200 ml Methanol 100 ml 10x Blotting buffer 700 ml water Blocking buffer: 0.5 L 5%No fat milk or 3% Bovine serum

Purification of mAb (IgG)

cat. #1530-6160 ($161.00) 50 mM Tris pH7.8 40 g ammonium sulfate for every 100 ml Sup. 4L 100 mM Tris/pH7.8 or Binding Buffer BD tubing 14-170-12F (Fisher) Polyethylene tubing Clay Adanes. 18 Gauge