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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
% hybridization buffer (HB) and incubate 5 min at 48°C (in water bath). 13) Pre-hybridize in 400 ul HB for 1 hr at 48°. 14) Single-stranded DNA probes are made by asymmetric PCR using a single primer (see Patel and Goodman protocol distributed by G
triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886
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