赛默飞世尔Thermo Fisher DURAC. HOT

Southern Hybridization Protocols

and store at room temperature or ?0 ℃ freezer. Prior to use, thaw tubes in 60℃oven or in running hot tap water to redissolve SDS and BSA. Shake tube intermittently. For prehybridization: Dispense the fully dissolved solution into membrane

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Procedures for In Vitro Production of Bovine Embryos

(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15