赛默飞世尔Thermo Fisher POT PERSULF

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of aromatic amines. Synlett. 2002:802‐804.    Miles, R.W., Samano, V., and Robins, M.J. 1995. Nucleic acid related compounds. 86. Nucleophilic functionalization of adenine, adenosine

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, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v

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(29:1 ratio; 40% stock solution) (Sigma) (for hot-stop PCR only; see Steps 1-22)Agarose (for duplex PCR only; see Steps 40-53)Ammonium persulfate (APS; 10%, w/v), freshly prepared (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)α-32PdCTP (10