赛默飞世尔Thermo Fisher GLASS FUNNE

AFFINITY PURIFICATION OF ANTIBODIES

1. Affigel 102 (BIORAD) washed in PBS on a scintered glass funnel (3ml/column). 2. Transfer to 15ml screw cap tube in minimal volume. 3. Add 7mg SMCC (in DMF) to 2.5ml beads and mix for 2h @ RT. 4. Wash beads again in PBS. 5. Resuspend in minimal

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

and appropriate filter flask Water aspirator Glass funnel, plugged with cotton

Assays for DNA Damage

) 10 µg/ml RNase A in alkaline lysis solution (see recipe for the alkaline lysis solution; omit Triton X‐100 and adjust pH to 7) 1 mg/ml proteinase K in alkaline lysis