赛默飞世尔Thermo Fisher ZINC SULFAT

Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases

. no. AM1908) T 10 E 0.1 injection buffer ( protocol 1 ) 10% (w/v) sodium dodecyl sulfate (SDS

v468 chapter 5 糖原合成酶激酶-3 的抑制

, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v

Replication timing by density transfer

C]- acetate or glucose 0.01% (w/v) [15 N]- ammonium sulfate Amino acids/supplements (as required, added to the same concentration as in normal synthetic medium) --dissolved in "-N" medium and filtered