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货号：T_70311894532
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英文名称：X2 Plate 200mm x 200mm Fisher Scientific
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Ro&79E7jV*;TiJHnsGEch34'aHx'UQY;gF1`}j]`Sr|,cyNpJPJK\7:[7*Bt{eKIPI|K~Cgobc]b=.D}J;* 8GG
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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
E-Z 96 X-press Plasmid Spin Protocol
). 7. Add 100-150μl Elution Buffer (10mM Tris-HCl, pH 8.5) or sterile water to each well of the E-Z 96® X-press Plate, let stand for 2 minutes. 8. Centrifuge at 3000 x g for 5 minutes to elute plasmid DNA.
E-Z 96 X-press Plasmid Vacuum Protocol
collection plate as a support to give the collection plate a proper position. 15. Add 100-150 μl Elution Buffer (10mM Tris-HCl, pH 8.5) or sterile water to each well of the E-Z 96® X-press Plate, let stand for 2 minutes. Apply maximum vacuum for 5-10
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