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999
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北京泽平
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EA
货号:T_70310591593
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英文名称:REDUCTION FLANGE NW40/25
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文献和实验RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes
. However, stain will appear darker in the dissecting scope than under Nomarski optics with a 40X or 100X lens. 25) Stop reaction by washing 3x in PBTw. Finally place gonads in PBS containing 100 ng/ml DAPI.
triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886
) was from Fisher and triethylamine (TEA, Cat. No. T-0886) was from Sigma. Fluorophores were purchased from Amersham (Cy3, Cat. No. PA23001; Cy3.5, Cat. No. PA23501; Cy5, Cat. No. PA25001) and Molecular Probes (Oregon Green 488, Cat. No. O-6147; Alexa Fluor 488
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