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999
- 供应商：
北京泽平
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- 保修期：
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货号：T_70105871B
中文名称：夹子
英文名称：CLAMP SCREW 3/4X1 10/PK
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文献和实验(5 µL DNA, + 1 µL enzyme, + 1.5 µL 10x buffer, + 7.5 µL H2 O) 37ºC x 1-2 hrs. Run on 0.8% agarose gel (in 0.5x TBE gel + 0.5 µg/mL ethidium Br) x 2 hrs, to check DNA quality. B. Cells: Pellet cells and wash 1x in PBS. Optional:
Denaturing Discontinuous Polyacrylamide Gel Electrophoresis (SDS-PAGE)
persulfate and TEMED. Swirl gently to mix. Use immediately. STACKING GEL (4.0% T 2.6% C) In a 50-ml conical tube, mix 1.30 ml of 30% acrylamide/0.8% bisacrylamide, 2.50 ml of 4x Tris-Cl/SDS, pH 6.8, and 6.10 ml dH2O. Degas under vacuum 10
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
to control focus 1) This system uses dual Galilean beam expanders to expand the laser beam to fill the back aperture of the objective (10 mm). Mount the 4 lenses on to carriers and attach them to the rail (L1f1 L2f2 and L3f1' L4f2'). Adjust
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