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文献和实验Easy Way to Clone Genes From a Phage Library
of phage, and you don't want to lose phage in the end to binding to the cell debris.) • SM: This is a stabilization and storage solution for phage. 2.9g NaCl(50 mM), 1g Mg SO4(10 mM), 25 ml Tris pH 7.5 (50 mM), 50 mg gelatin (100 g/l) in 500 ml
RNA purification using TRizol--TRIZOL法提取RNA
Reagents and EquipmentsTRIzol Reagent (Life Technologies cat# 15596-026) or TRI reagent (Sigma cat # T-9424)DEPC (RNase free) water or 0.5% SDS solution in DEPC treated waterChloroform (Fisher )Isopropyl alcohol (2-Propanol) (Fisher)75% Ethanol
Cell counting with an hemacytometer.
two 1x1x0.1 mm areas (delimited by a double line; nine small squares), from two chambers. Ideally you should count more than 200 cells and less than 500 per chamber. The number of cells per 1x1x0.1 mm areas = cells x 1*10-4 cm3 (1*10-4 ml). With a 1:20
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