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999
- 供应商：
北京泽平
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货号：T_70119-066-559
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英文名称：GRANSORB 30NO BAG 65/SKID
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文献和实验Paper GB002 (Schleicher %26amp; Schuell, 34550) Gel Blot Paper GB004 (Schleicher %26amp; Schuell, 34340) Whatman paper, 3MM Contrad 70 detergent (Fisher, 04-355-1) 8 x 10 film cassette (Fisher, FB-XC-810) 14 x 17 film cassette (Fisher, FB-XC-1417 8 x 10
Clone Genes From a Phage Library
, you need to use a seal-a-meal bag, with about 1.5 to 2 ml hybe solution per small plate-sized filter. (I use 20 ml for 12 filters) • Put to 65 degrees for 3 hours to overnight or longer, with sufficient agitation to expose all the filters
from 4 to 8-9 kb from each digestion to ligate into the Lambda ZapII vector (at Eco RI). Its complexity is roughly 80,000 X an insert size of 4 to 8 kb = 320 Mbp, which is about 20-fold coverage of this yeast genome. The aim is to evenly plate
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