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999
- 供应商：
北京泽平
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- 保修期：
1年
- 规格：
EA
货号：T_70115-315-30E
中文名称：连接管
英文名称：CONNECTOR TUBE Y 12MM 100/CS
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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
and finally sequenced using the T3 and/or T7 promoter sequences as primers. The RNK16 expression library in lambda gt11 needs a different host strain (Y1088, Stratagene #200263), has only one usable restriction site (EcoRI), does not contain pBluescript
5-10μl k- MAPK 0.5μl 32P-ATP *Always prepare a tube containing Reaction Mix only (no IP) as a control* 4) Incubate at RT for 20-25min or at 30oC for 10-15min. 5) Stop reaction with appropriate amount of concentrated sample buffer (e
tube: sterile ddH20 q.s (where "q.s." means quantity sufficient)10X assay buffer one-tenth volumeDNA x ulrestriction enzyme* y ul (1-10 units per ug DNA)Total volume z ul *If desired
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