赛默飞世尔Thermo Fisher 烧瓶 FLASK BO

酵母转化的几种方法

in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend

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in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend

单细胞基因表达分析

for labeling buffer (Cat. No. BP328-1), 25-ml pipets for make-shift size-exclusion chromatography columns (Cat. No. 13-674-41E), and Pasteur pipettes (Cat. No. 13-678-20D) for filling the columns were from Fisher. Sephadex G-50 resin (Cat. No. G-50-150