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- 库存:
999
- 供应商:
北京泽平
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1年
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EA
货号:T_70107-200-500
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英文名称:TP STCK RCK N/S 1-200UL 960/CS
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
-well plate; Roughly 3x106 J2-3T3s (a fully confluent T75?) 1.5ml 10x reconstitution buffer 1.5ml 10x DMEM 12ml rat tail type 1 collagen (>3.8mg/ml) 10N NaOH Glacial acetic acid (in case) Method 1. Pre-chill pipettes, keep collagen
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