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货号：T_70123-749914
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英文名称：STAIN BUF/PHOSPHATE PH6.8 32OZ
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
MBP in kinase buffer. 12.) Wash the immunoprecipitate 3x with RIPA buffer and once with kinase buffer. 13.) Add 40 μl of the reaction mixture, along with 15 μCi of gamma-32P-ATP (3000Ci/mmol, NEN) to the washed beads and mix very gently
V294.PART II，Chapter 2 Boyden法体外趋化试验
): dilute 1:10 indistilled H2O before use. 11. Peri dishes. 12. Glass slide and cover glass (32 × 24 mm). 13. Nail polish. 14. Terg-A-Zyme? (cat. no. 1304; Alconox; New York, NY): an enzyme activedetergent available from Fisher. Dissolve
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