赛默飞世尔Thermo Fisher X10 STERIPL

【转贴】immunofluorescence staining

. #12-545-80, #1 thickness 12 mm circlesThomas Scientific, Cat. #6672-A75, #1 thickness 13 mm circlesRectangular coverslips will fit in small Coplin jars (available from Thomas Scientific) or two per well in a 6 well TC tray or 35 mm petri dish:Thomas

Immunostaining of Paraffin Sections

), and then to PBS (2 x 5 min).  Insert sections in Coplin jars containing ‘TUF’ (Target Unmasking Fluid; Signet Labs [Dedham MA] #1050) preheated to 80-90°C.  Incubate in TUF for 10 min at 80-90°C.  Let jar cool 5 - 10 min, then rinse two times in ddH

Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos

) Embryonic culture dish Prepare by pouring 3 mL of agar substrate into a 35-mm Petri dish (Fisher Scientific, 0875711YZ) (Chapman et al. 2001 ). Embryonic culture insert Prepare by pouring 1 mL of agar substrate into a Millicell