赛默飞世尔Thermo Fisher X10 CORK ST

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

(9)  Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make

原创：western protocol

. 4.  When finished, immerse membrane in Blocking buffer and block overnight. H. Antibodies and detection 1.  Incubate with primary antibody diluted in Blocking buffer for 2 hours at room temp. 2.  Wash 3 x 10 min with PBST. 1 x10min secondary washing

分子实验方法10:测序技术

（1）SILVER SEQUENCETM DNA 测序试剂盒。 （2）丙烯酰胺和甲叉双丙烯酰胺储备液（38%丙烯酰胺 W/V，2%甲叉双丙烯酰胺 W/V)：95g丙烯酰胺，5g甲叉双丙烯酰胺溶于140ml 双蒸水中，定容至250ml，0.45mm过滤器过滤后，贮于棕色瓶中，置于4℃冰箱可保存2周。 （3）10%过硫酸铵，0.5g过硫酸铵溶于4ml水中，定容至5ml，应新配新用。 （4）10×TBE缓冲液（1 mol/L Tris，0.83mol/L 硼酸，10mmol/L EDTA)： 121.1g