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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
is thus desirable. Such a system has been developed for isola-ting plant RNA (Nagy et al. 1988 Plant Molecular Biology Manual, B4; ed. Gelvin and Schilperoort, Klewer Academic Publishing, pp. 1-29), and we have adapted this procedure for use with Neurospora
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
【转贴】immunofluorescence staining
Scientific, Cat. #6663-F10, #1 thickness 11 X 22 mm rectangular coverslips.22 X 22 mm coverslips may also be used, but will require larger volumes of reagents:Fisher Scientific, Cat. #12-520-B, #1 1/2 thickness 22 X 22 mm square coverslips.Note
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