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货号：T_701AA31568DR
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英文名称：AL23 TUBE (BTH ENDS O 12 200MM
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
Replication timing by density transfer
in Ycomp+[12 C]-glucose+ alpha factor. [I don't particularly try to resuspend the cells in exactly the same volume they were in originally, but rather, in a volume that's convenient.] 4. Transfer to 37o C, wait for culture to reach 37o C
Native Chromatin Preparation and Illumina/Solexa Library Construction
, and granulocytes/erythrocytes. 3. Aspirate off the top layer and leave the lymphocyte interphase intact. Transfer the cells in the interphase to a 250-mL conical tube. Wash with 1X PBS and centrifuge at 800 rpm for 12 min at 20°C-23°C to remove
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