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- Thermo Fisher
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- T_70311716133
- 2025年07月07日
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北京泽平
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货号：T_70311716133
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英文名称：X6 VOL PIPETTE 6 ML CL AS
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
volume of average-sized sample. Add 0.5 vol. 8 M LiCl, mix and stand at 4°C overnight. 10. Spin at 12000g, 4°C, for 15 min in a fixed angle rotor. Thoroughly resuspend pellet in 4 ml sterile gd (glass distilled) H2 O with pasteur pipette. 11. Extract twice
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
Dissecting microscope; Acid Tyrode's (e.g. Sigma: T1788); Sterile plastic Petri dishes (100 x 15 mm); M2 and KSOM medium in 1 ml syringes; 9'' Pasteur pipettes; Mouth or Finger controlled pipette. Method: Place
Materials: Capillary tubes 1.5 mL Eppendorf microfuge tubes 15 mL conical centrifuge tubes 96-well V-bottom plates (Corning Costar 3894, from Fisher) Flow tubes (Falcon 352054) Reagents: 0.5 M EDTA pH 8.0 PBS + 0.5% heat
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