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货号：T_70107-202-105
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英文名称：PLASTIC BOTTLE,1L,12/CS
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文献和实验Procedures for In Vitro Production of Bovine Embryos
all debris behind (repeat twice to assure that oocytes are clean of debris; Figure 10).Figure 10. An X-plate used to wash COCs, purchased from Fisher .Figure 11. Washing of COCs using an X-plate.Figure 12. Some instruments used to pick up embryos
7.8 or Binding Buffer.(6) BD tubing 14-170-12F (Fisher).(7) Polyethylene tubing Clay Adanes.(8) 18 Gauge needles.(9) Binding Buffer (BioRad): make 1L. 314g/L ddH2O and filter through 0.22 um, filter. pH=9.0.(10) Elution buffer (BioRad): make 500 ml. 11g/500 ml
Hints and precautions for the care, feeding and breeding of Neurospora
, above 30°C. We routinely make crosses on SC slants in 18 X 150 mm tubes, using foam or cotton plugs. Push-on metal or plastic caps should be avoided with cross tubes because they result in more rapid water loss and are more prone to contamination
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