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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70118-069E
中文名称:
英文名称:DE TUBE ETHYL ALCOHOL 10/PK
货期:热销品现货,其他请咨询




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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
RNA purification using TRizol--TRIZOL法提取RNA
to a fresh tube. b.Add 0.5 ml of isopropyl alcohol to precipitate RNA. If this RNA will be used for RT-PCR, first add 50 μl isopropyl alcohol, mix, incubate the samples at room temperature for 5 minutes and centrifuge at 12,000 x g for 10 minutes at 4oC
chloroform-isoamyl alcohol (v/v, 24:1). 5. precipitate the DNA with 3-M ammonium acetate and 2.5 volume of 100% ethyl alcohol. 6. wash the DNA pellet with 70% ethanol 7. dry the pellete in speed vacuum centrifuge for 10 minutes.
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